9PNX
Crystal structure of anti-IL2Rgamma VH3-20 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 |
| Unit cell lengths | 46.821, 46.821, 53.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.110 - 1.350 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.955 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.110 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.062 | 0.869 |
| Number of reflections | 25124 | 1079 |
| <I/σ(I)> | 36.5 | 1.3 |
| Completeness [%] | 98.5 | 84.6 |
| Redundancy | 6.1 | 5.4 |
| CC(1/2) | 0.995 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M CaOAc, 0.1 M MES pH 6.0, 20% PEG 8000 |
