9PNK
Crystal structure of the GH29-like fucosidase FucWf4 in complex with fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.785, 97.475, 123.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.880 - 2.190 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.847 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 2.260 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 52606 | 4280 |
| <I/σ(I)> | 7.2 | 1.8 |
| Completeness [%] | 99.1 | |
| Redundancy | 8.4 | |
| CC(1/2) | 0.994 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 18% PEG 6000, 0.12 M sodium acetate, 0.1 M Tris pH 8.5 |
