9PM4
The structure of O-glycopeptidase BcM60C from Bacteroides caccae in complex with a 6-sialo core 1 glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-16 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.543, 118.543, 90.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.800 - 1.550 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.20550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmeas | 0.056 | 0.056 |
| Rpim | 0.022 | 0.267 |
| Number of reflections | 93659 | 4620 |
| <I/σ(I)> | 27.8 | 2.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.997 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M (NH4)2 SO4, 0.1M Na citrate pH5.6, 21% PEG4000 |
