9PLL

TRIM21-NUP98 Molecular Glue Complex (MAN-056)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	99.5
Detector technology	PIXEL
Collection date	2025-05-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979460
Spacegroup name	P 21 21 21
Unit cell lengths	57.481, 71.307, 97.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.510 - 1.600
Rwork	0.175
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.692
Data reduction software	xia2 (0.6.477)
Data scaling software	Aimless
Phasing software	PHENIX (AutoMR (1.21.2))
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	1.600
High resolution limit [Å]	1.560	1.560
Rmerge	0.128
Number of reflections	57407	2696
<I/σ(I)>	7.4
Completeness [%]	99.4
Redundancy	6.1
CC(1/2)		0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	100 mM NaCl, 100 mM HEPES, pH 7.5, 12 % polyethylene glycol 20,000