9PIW

Crystal structure of a synthetic Fab (1A) in complex with the FRB domain of mTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	64.938, 64.983, 80.302
Unit cell angles	70.35, 68.34, 71.13

Refinement procedure

Resolution	33.630 - 1.900
R-factor	0.2237
Rwork	0.222
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.849
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.640	1.960
High resolution limit [Å]	1.900	1.900
Number of reflections	86739	2889
<I/σ(I)>	7.2
Completeness [%]	97.5
Redundancy	2
CC(1/2)	0.997	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M ammonium citrate dibasic, 20% PEG 3350