9PI4

Poly dT bound form of Single stranded DNA-binding protein(ICP8) from Herpes simplex virus-1. Mutations: C254S, C455S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97933
Spacegroup name	P 32 2 1
Unit cell lengths	151.736, 151.736, 154.094
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.510 - 3.100
R-factor	0.19501
Rwork	0.193
R-free	0.23274
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.518
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.060	3.240
High resolution limit [Å]	3.100	3.100
Rmerge	0.118
Rpim		0.658
Number of reflections	37723	4546
<I/σ(I)>	7.1	1.3
Completeness [%]	100.0	100
Redundancy	16.6	17.4
CC(1/2)	0.998	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M ammonium acetate, 0.02 M magnesium chloride, 0.05 M HEPES, pH 7.0, 5% PEG8000