9PHP
[19-7B G|A] 19 bp tensegrity triangle that propagates via blunt-end stacking with G stacking on A at the interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.007430 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 157.020, 157.020, 157.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.480 - 4.330 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.659 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.103 | 64.103 | 4.591 |
| High resolution limit [Å] | 4.328 | 10.445 | 4.328 |
| Number of reflections | 2217 | 200 | 201 |
| <I/σ(I)> | 31.4 | ||
| Completeness [%] | 92.3 | ||
| Redundancy | 67.5 | ||
| CC(1/2) | 1.000 | 1.000 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
