9PH0

Crystal structure of MAR1 from Gelatoporia subvermispora

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-31
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	67.660, 73.930, 101.960
Unit cell angles	97.55, 107.77, 90.01

Refinement procedure

Resolution	37.990 - 1.790
R-factor	0.1645
Rwork	0.162
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.990	37.990	1.840
High resolution limit [Å]	1.790	8.010	1.790
Rmerge	0.129	0.081	0.673
Rmeas	0.144	0.089	0.819
Number of reflections	169444	1949	11675
<I/σ(I)>	6.98
Completeness [%]	96.4
Redundancy	4.4
CC(1/2)	0.993	0.991	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.12M Ethylene glycols, 0.1M Buffer system 3 (pH 8.5) and 37.5% Precipitant Mix 4