9PFO

Structure of POU2F3 POU domains bound to coactivator OCA-T2 and DNA (2.1 angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	P 1 21 1
Unit cell lengths	79.019, 95.802, 85.637
Unit cell angles	90.00, 101.24, 90.00

Refinement procedure

Resolution	29.830 - 2.100
R-factor	0.2211
Rwork	0.219
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	XDS (February 5, 2021)
Data scaling software	pointless (1.12.10)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.850	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.246	0.676
Rmeas	0.303	0.830
Rpim	0.173	0.473
Total number of observations	224565	14046
Number of reflections	71525	4421
<I/σ(I)>	4.6	2.9
Completeness [%]	97.9
Redundancy	3.1	3.2
CC(1/2)	0.872	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1 M MMT (1:2:2 molar ratio of DL-malic acid:MES:Tris base), pH 6.0, 25% PEG-1500