9P8Z
Crystal Structure of GTP cyclohydrolase 1 (FolE) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-25 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.547, 109.121, 122.548 |
| Unit cell angles | 89.11, 88.12, 77.95 |
Refinement procedure
| Resolution | 48.760 - 2.740 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.832 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((2.0_5723: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 2.810 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.120 | 0.815 |
| Rmeas | 0.143 | 0.963 |
| Rpim | 0.076 | 0.506 |
| Total number of observations | 394766 | 29753 |
| Number of reflections | 112173 | 8349 |
| <I/σ(I)> | 9.5 | 1.9 |
| Completeness [%] | 96.3 | |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.992 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | JCSG+ B8: 0.2M Magnesium Chloride hexahydrate, 0.1M Tris pH 7.0, 10% PEG 8K. MytuD.00091.a.A1.PW39335 at 8.3 mg/mL. plate 19669 well B8 drop1, Puck: PSL-1009, Cryo: 80% crystallant + 20% PEG 200 |
