9P33
Crystal structure of the Bromo domain 1 in human Bromodomain Containing Protein 4 with a compound
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.803, 48.464, 61.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.604 - 1.010 |
| R-factor | 0.1404 |
| Rwork | 0.139 |
| R-free | 0.16020 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.604 | 1.059 |
| High resolution limit [Å] | 1.010 | 1.010 |
| Rmerge | 0.071 | 1.088 |
| Rmeas | 0.080 | 1.277 |
| Rpim | 0.036 | 0.652 |
| Number of reflections | 62745 | 2930 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M NaNO3, 20% PEG3350, 10% Ethylene glycol, pH 7.5 |
