9P1W
Crystal structure of hen egg white lysozyme at 100 Kelvin with Lard (Triplicate)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER TURBO X-RAY SOURCE |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-01 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.253, 78.253, 37.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.746 - 1.497 |
| Rwork | 0.212 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.839 |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.746 | 10.000 |
| High resolution limit [Å] | 1.497 | 1.497 |
| Number of reflections | 19188 | 19188 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.7 | |
| Redundancy | 1 | |
| CC(1/2) | 0.900 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 1.2 M sodium chloride, 100 mM sodium acetate, pH 4.6 |
