9P1H
100K human S-adenosylmethionine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-20 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.855, 55.946, 99.247 |
| Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
| Resolution | 43.460 - 1.810 |
| R-factor | 0.1816 |
| Rwork | 0.181 |
| R-free | 0.19930 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.816 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.480 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.074 | 1.671 |
| Rmeas | 0.088 | 1.983 |
| Rpim | 0.047 | 1.054 |
| Total number of observations | 225636 | |
| Number of reflections | 67064 | 5042 |
| <I/σ(I)> | 9.4 | 1.2 |
| Completeness [%] | 96.9 | 98 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.998 | 0.301 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 4mg/mL enzyme in 200mM NaCl 50mM HEPES pH 7.5 1:1 to reservoir solution containing 4-8% PEG 8000 and 50mM Tris-HCl pH 8.5 |
