9P0Z

Crystal structure of hen egg white lysozyme at 100 Kelvin with Apiezon N (Duplicate)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-01
Detector	Bruker PHOTON II
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	78.345, 78.345, 37.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.001 - 1.499
Rwork	0.206
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.854
Data reduction software	SAINT
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.001	10.000
High resolution limit [Å]	1.499	1.500
Number of reflections	19135	19135
<I/σ(I)>	16
Completeness [%]	99.0
Redundancy	20
CC(1/2)	0.900	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.2 M sodium chloride, 100 mM sodium acetate, pH 4.6