9P0P

Crystal structure of 23S rRNA methyltransferase Cfr, apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	45.319, 58.612, 115.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.210 - 2.500
R-factor	0.2568
Rwork	0.254
R-free	0.30930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MrBUMP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.075	0.882
Rpim	0.027	0.325
Number of reflections	10556	401
<I/σ(I)>	32.42	1.6
Completeness [%]	94.1	72.6
Redundancy	9	7.3
CC(1/2)		0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	40% v/v PEG400, 0.1 M HEPES pH 7.5, 7 mM beta-mercaptoethanol, 0.1 M NDSB211