9OZX

Crystal structure of ZNRF3 RING in complex with UbcH5b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	H 3 2
Unit cell lengths	153.653, 153.653, 142.103
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.050 - 2.400
R-factor	0.2495
Rwork	0.249
R-free	0.26570
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.050	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	48893	2494
<I/σ(I)>	27.32
Completeness [%]	99.9
Redundancy	14
CC(1/2)	0.998	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	1.7 M ammonium sulfate, 0.1 M sodium acetate, pH 5.0