9OZD
Crystal structure of the polysaccharide lyase RbmB from Vibrio cholerae bound to Vibrio Polysaccharide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979361 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.555, 52.563, 133.229 |
| Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
| Resolution | 33.420 - 1.620 |
| R-factor | 0.1699 |
| Rwork | 0.168 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.676 |
| Data reduction software | XDS (Jun 30, 2023 (BUILT 20230630)) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.424 | 1.786 |
| High resolution limit [Å] | 1.618 | 1.618 |
| Rmerge | 0.127 | 0.759 |
| Rmeas | 0.151 | 0.911 |
| Rpim | 0.105 | 0.497 |
| Number of reflections | 131400 | 6572 |
| <I/σ(I)> | 7 | 1.6 |
| Completeness [%] | 92.6 | 61.9 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.996 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.4M Ammonium formate, 20% PEG 3,350 |
