9OWI

Structure of the A. thaliana TBP bound to TBP-AG nicked DNA site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	68.557, 117.295, 102.138
Unit cell angles	90.00, 107.00, 90.00

Refinement procedure

Resolution	48.290 - 2.480
R-factor	0.2041
Rwork	0.202
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.520
High resolution limit [Å]	2.480	2.480
Rmerge	0.086	0.550
Rpim	0.055	0.342
Number of reflections	54270	4434
<I/σ(I)>	9	2.2
Completeness [%]	99.1
Redundancy	3.5
CC(1/2)	0.995	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 3350, 0.1 M Citric acid pH 5.0 and 0.2 M sodium citrate.