9OIQ

The von Hippel Lindau-ElonginB-ElonginC (VCB) complex with fragment 15

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 41 2 2
Unit cell lengths	94.281, 94.281, 363.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.150 - 2.660
R-factor	0.2287
Rwork	0.226
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.150	2.750
High resolution limit [Å]	2.660	2.660
Rmerge	0.037	0.277
Number of reflections	92676	92676
<I/σ(I)>	18.9	2.8
Completeness [%]	100.0	100
Redundancy	1.9	1.9
CC(1/2)	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium cacodylate pH 5.5, 0.2 M magnesium acetate, 5 mM DTT and 14-20% PEG8000