Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000050 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.684, 70.640, 84.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.127 - 1.286 |
| Rwork | 0.186 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.749 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.127 | 1.336 |
| High resolution limit [Å] | 1.286 | 1.290 |
| Number of reflections | 80248 | 5110 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 93.2 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.996 | 0.333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 21% PEG3350, 0.36 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5 |
