9OFS

Crystal structure of the human IGA2m2 FC fragment-FC-alpha receptor (CD89) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	46.637, 128.955, 183.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.690 - 1.950
R-factor	0.2287
Rwork	0.227
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.937
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.118
Rpim	0.049	0.437
Number of reflections	37981	1500
<I/σ(I)>	17
Completeness [%]	93.4	76.3
Redundancy	6.2	5.4
CC(1/2)	0.997	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.15M sodium chloride 0.1M Tris-HCl pH 8.0 20% PEG 1000