9ODX

Structure of Unc119B in complex with N-acetyl-S-geranylgeranyl-L-Cysteine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-11
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	80.670, 62.641, 51.872
Unit cell angles	90.00, 117.74, 90.00

Refinement procedure

Resolution	47.090 - 1.450
R-factor	0.1585
Rwork	0.157
R-free	0.19420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.525
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.090	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.059	0.682
Rmeas	0.069	0.803
Rpim	0.036	0.420
Number of reflections	40180	1983
<I/σ(I)>	15.4	2.2
Completeness [%]	99.2	99.2
Redundancy	6.9	7.1
CC(1/2)	0.998	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M bis-tris propane/tris, 1.5-2.2 M Ammonium sulphate, pH 5.5-8.5