9OD9

Structure of disulfide-stabilized IL-18 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 61
Unit cell lengths	70.821, 70.821, 88.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.930 - 1.600
R-factor	0.1713
Rwork	0.171
R-free	0.18780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.898
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3-4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.440	1.627
High resolution limit [Å]	1.600	1.600
Rmerge	0.047
Number of reflections	32207	1652
<I/σ(I)>	22.9
Completeness [%]	96.7
Redundancy	10.4
CC(1/2)	1.000	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M sodium citrate, pH 5.0, 20% PEG 600