9OCW

A constitutively active construct of eukaryotic elongation factor 2 kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920221
Spacegroup name	P 1 21 1
Unit cell lengths	64.766, 59.736, 88.766
Unit cell angles	90.00, 98.50, 90.00

Refinement procedure

Resolution	49.390 - 2.270
R-factor	0.2027
Rwork	0.200
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	XDS (VERSION Jan 19, 2025 BUILT=20250327)
Data scaling software	XSCALE
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	2.352
High resolution limit [Å]	2.270	2.271
Rmeas	0.235
Rpim	0.088	0.503
Number of reflections	17519	876
<I/σ(I)>	6.6	1.6
Completeness [%]	91.7	63
Redundancy	7	6.9
CC(1/2)	0.994	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	2 ul of protein were mixed with 1 ul of a 18.8% PEG3350 (Hampton Research) and 314 mM magnesium acetate (pH not adjusted) solution in a 24 wells plate at room temperature