9O6N

Structure of Siglec-10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97741
Spacegroup name	P 65 2 2
Unit cell lengths	83.830, 83.830, 484.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.500 - 2.980
R-factor	0.2166
Rwork	0.212
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.222
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.440	48.440	3.060
High resolution limit [Å]	2.980	13.330	2.980
Rmerge	0.271	0.051	1.830
Rmeas	0.279	0.053	1.883
Number of reflections	21921	324	1560
<I/σ(I)>	10.84
Completeness [%]	99.9
Redundancy	18.3
CC(1/2)	0.997	0.999	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.085 M Sodium Citrate pH 5.4, 0.17 M Ammonium Acetate, 15% v/v Glycerol, and 23% w/v PEG 4000