9O63

Crystal structure of PLK4 and RP1664 complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	63.140, 83.890, 52.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.540 - 2.260
R-factor	0.2151
Rwork	0.213
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.624
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.540	44.540	2.400
High resolution limit [Å]	2.260	6.710	2.260
Rmerge	0.121	0.034	1.444
Rmeas	0.163	0.044	1.631
Number of reflections	25230	304	1341
<I/σ(I)>	3.87
Completeness [%]	99.7
Redundancy	4.6
CC(1/2)	0.992	0.997	0.057

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	9 - 14% PEG4K, 0.1 M Na Cit; pH 5.4 - 5.5, 0.2 M (NH4)2SO4