9O3D
Crystal structure of broadly neutralizing antibody HEPC108 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.640, 78.530, 227.781 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 52.320 - 2.680 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.210 | 2.720 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.144 | 1.772 |
| Rmeas | 0.157 | 1.927 |
| Rpim | 0.062 | 0.747 |
| Total number of observations | 704440 | 35924 |
| Number of reflections | 112353 | 5576 |
| <I/σ(I)> | 8.9 | 1.2 |
| Completeness [%] | 96.6 | |
| Redundancy | 6.3 | 6.4 |
| CC(1/2) | 0.996 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 0.2 M Potassium sodium tartrate tetrahydrate, 20% PEG3,350 |
