Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-12-15 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.807, 93.855, 153.660 |
| Unit cell angles | 89.73, 89.68, 75.79 |
Refinement procedure
| Resolution | 45.490 - 2.416 |
| R-factor | 0.1878 |
| Rwork | 0.186 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4 (8-JUN-2022)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.460 |
| High resolution limit [Å] | 2.416 | 6.560 | 2.420 |
| Rmerge | 0.072 | 0.022 | 0.922 |
| Rmeas | 0.084 | 0.026 | 1.071 |
| Rpim | 0.042 | 0.013 | 0.542 |
| Number of reflections | 165005 | 8330 | 8245 |
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 98.6 | 99.3 | 97.8 |
| Redundancy | 3.9 | 3.8 | 3.8 |
| CC(1/2) | 0.991 | 0.997 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 5 uL of protein complex solution and 5 uL of well solution containing 0.1 M MES pH 6.5 and 25% polyethylene glycol 6000. |
