9NYM
Crystal structure of an MKP5 allosteric loop mutant, N448A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-17 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.310, 40.531, 57.380 |
| Unit cell angles | 74.62, 78.86, 61.55 |
Refinement procedure
| Resolution | 34.910 - 2.000 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.857 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18_3845: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.910 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | 0.156 |
| Number of reflections | 18191 | 1464 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 89.9 | |
| Redundancy | 1.7 | |
| CC(1/2) | 0.981 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1 M Tris, pH 6.5, 2.0 M ammonium sulfate |
