9NXF

Crystal structure of CN:Tak1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920105
Spacegroup name	C 1 2 1
Unit cell lengths	224.079, 49.585, 158.895
Unit cell angles	90.00, 128.72, 90.00

Refinement procedure

Resolution	34.940 - 3.130
R-factor	0.2503
Rwork	0.248
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.400
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.940	3.184
High resolution limit [Å]	3.130	3.130
Number of reflections	23625	1213
<I/σ(I)>	3.5	0.9
Completeness [%]	96.0	100
Redundancy	6.8	7.2
CC(1/2)	0.935	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	298	200 mM Sodium citrate tribasic pH 8.4, 22% PEG 3350, 3% ethanol. Microseeding