9NW7

CA117v2v8 Fab bound to HLA-E-VL9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	57.540, 71.840, 121.210
Unit cell angles	90.00, 102.01, 90.00

Refinement procedure

Resolution	32.990 - 2.100
R-factor	0.1907
Rwork	0.189
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.840	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	55753	4546
<I/σ(I)>	5	1.4
Completeness [%]	98.6
Redundancy	3.4
CC(1/2)	0.989	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 25% PEG 3350