9NUG
D-Ornithine/D-lysine decarboxylase C387A complexed with putrescine, D-arginine and agmatine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-11 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.025, 49.087, 139.365 |
| Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
| Resolution | 62.690 - 1.980 |
| R-factor | 0.1939 |
| Rwork | 0.191 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.653 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.690 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 48691 | 818 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 80.9 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.991 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES, pH 7.5, 20% PEG 3350, 0.2-0.4 M sodium acetate |
