9NTJ
Chimeric Adenosine deaminase growth factor (ADGF) in complex with pentostatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.349, 109.609, 171.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.690 - 3.280 |
| R-factor | 0.2125 |
| Rwork | 0.210 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.522 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 3.410 |
| High resolution limit [Å] | 3.280 | 3.290 |
| Rpim | 0.123 | 0.622 |
| Number of reflections | 18753 | 1881 |
| <I/σ(I)> | 3.1 | 1.1 |
| Completeness [%] | 93.4 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.803 | 0.331 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 25% w/v Polyethylene glycol 1,500, 15% Glycerol |
