9NRT

Crystal structure of the H5 influenza virus hemagglutinin from A/duck/France/161108h/2016 (H5N8) clade 2.3.4.4b in complex with O-linked glycan 25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-31
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	95.170, 171.615, 226.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.870 - 1.980
R-factor	0.1657
Rwork	0.165
R-free	0.18880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.856
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.870	2.010
High resolution limit [Å]	1.980	1.980
Rpim	0.050	0.670
Number of reflections	254864	11594
<I/σ(I)>	16.1	0.9
Completeness [%]	98.7
Redundancy	6.7
CC(1/2)	0.995	0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris, pH 7.0, 0.11 M lithium sulfate and 2.0 M ammonium sulfate