9NRB
Crystal structure of H5 hemagglutinin Q226L mutant from the influenza virus A/duck/France/1611008h/16 with LSTc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.915, 176.398, 226.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.810 - 2.650 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.555 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.810 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmeas | 0.290 | 2.900 |
| Rpim | 0.085 | 0.850 |
| Number of reflections | 110745 | 10532 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 11.4 | |
| CC(1/2) | 0.990 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris, 2M ammonium sulfate, 0.2M lithium sulfate |
