9NR5
Crystal structure of H5 hemagglutinin Q226L mutant from the influenza virus A/black swan/Akita/1/2016 with LSTc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.080, 99.827, 132.059 |
| Unit cell angles | 90.00, 126.19, 90.00 |
Refinement procedure
| Resolution | 39.680 - 2.520 |
| R-factor | 0.2032 |
| Rwork | 0.200 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.567 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.680 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmeas | 0.127 | 0.950 |
| Rpim | 0.049 | 0.360 |
| Number of reflections | 65184 | 6297 |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 98.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M ammonium format, 20% PEG3350 |
