9NQX
Human Neu2 Sialidase Engineered for Better Half-Life
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 32 |
| Unit cell lengths | 91.639, 91.639, 135.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.500 - 1.890 |
| R-factor | 0.20597 |
| Rwork | 0.203 |
| R-free | 0.27057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.391 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.500 | 10.000 |
| High resolution limit [Å] | 1.890 | 2.110 |
| Rmeas | 0.090 | |
| Rpim | 0.038 | |
| Number of reflections | 39430 | 39430 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 93.3 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 6000 8% w/v, NaCl 1.75M |
