9NK5

[0,7,12-->14] Shifted tensegrity triangle with an (arm,center,arm) distribution of (0,7,12) base pairs and 2 nt sticky ends containing a semi-junction that becomes left-handed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000000
Spacegroup name	H 3
Unit cell lengths	112.776, 112.776, 68.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.680 - 4.580
R-factor	0.1966
Rwork	0.195
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.748
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.682	39.682	5.149
High resolution limit [Å]	4.580	9.406	4.584
Number of reflections	1459	208	210
<I/σ(I)>	10.5
Completeness [%]	86.2
Redundancy	10.3
CC(1/2)	0.997	0.997	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate