9NFR
Crystal structure of CRBN-DDB1 and MRT-23227 in complex with VAV1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95375 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 143.599, 143.599, 367.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.450 - 3.400 |
| R-factor | 0.2136 |
| Rwork | 0.211 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.329 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_5430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.580 | 3.510 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.336 | |
| Number of reflections | 31052 | 2011 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 98.2 | |
| Redundancy | 38.2 | |
| CC(1/2) | 0.998 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.6 % PEG Smear Low, 6.1 % PEG Smear Medium, 4.3 % PEG Smear High, 5 % glycerol, 0.1 M CaCl2, and 0.1 M MES pH 5.8 |
