9NDI

[3,8,9,P-1] Shifted tensegrity triangle with an (arm,center,arm) distribution of (3,8,9) base pairs, 1 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	P 63
Unit cell lengths	133.081, 133.081, 49.815
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 6.390
R-factor	0.1619
Rwork	0.161
R-free	0.16730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.902
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.960	19.956	7.109
High resolution limit [Å]	6.390	10.939	6.393
Number of reflections	968	194	194
<I/σ(I)>	6.9
Completeness [%]	90.6
Redundancy	19.5
CC(1/2)	0.992	0.991	0.046

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate