9NDB
[4,7,8-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (4,7,8) base pairs and 2 nt sticky ends
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000010 |
| Spacegroup name | H 3 |
| Unit cell lengths | 108.119, 108.119, 90.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.960 - 4.070 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.17280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.733 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.091 | 65.091 | 4.518 |
| High resolution limit [Å] | 4.070 | 10.261 | 4.074 |
| Number of reflections | 2180 | 198 | 197 |
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 86.7 | ||
| Redundancy | 10.7 | ||
| CC(1/2) | 1.000 | 1.000 | 0.348 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
