9NBZ

[5,7,7-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (5,7,7) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.007430
Spacegroup name	H 3
Unit cell lengths	106.336, 106.336, 91.072
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.510 - 3.980
R-factor	0.2185
Rwork	0.216
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.720
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.754	64.754	4.283
High resolution limit [Å]	3.977	10.226	3.977
Number of reflections	2570	197	195
<I/σ(I)>	15.9
Completeness [%]	89.2
Redundancy	10.8
CC(1/2)	0.999	0.999	0.157

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate