9N9Y

Crystal structure of truncated USP1:UAF1 in complex with compound 18

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.18064
Spacegroup name	I 2 2 2
Unit cell lengths	123.200, 161.880, 160.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.430 - 3.150
R-factor	0.2399
Rwork	0.237
R-free	0.29150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.475
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.430	49.430	3.370
High resolution limit [Å]	3.150	8.910	3.150
Rmerge	0.095	0.031	0.434
Number of reflections	13245	1339	193
<I/σ(I)>	12.1	36.5	3.6
Completeness [%]	46.4
Redundancy	6.4	5.9
CC(1/2)	0.999	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	1M sodium citrate (pH 6.5)