9N8N

Tandem antigen chimera of Pfs230 and Pfs48/45 bound by potent mAbs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.03322
Spacegroup name	C 1 2 1
Unit cell lengths	227.990, 80.490, 137.730
Unit cell angles	90.00, 111.46, 90.00

Refinement procedure

Resolution	70.100 - 2.220
R-factor	0.1753
Rwork	0.174
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.245
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.260	2.450
High resolution limit [Å]	2.220	2.220
Rpim	0.051	0.499
Number of reflections	81183	4058
<I/σ(I)>	12.2	1.7
Completeness [%]	93.6
Redundancy	16.1
CC(1/2)	0.998	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Na Cacodylate pH 6.5, 40% PEG 300, 0.2 M Ca acetate, 15% ethylene glycol