9N3R

Crystal structure of PRMT5:MEP50 in complex with MTA and TNG462

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 21 21 2
Unit cell lengths	98.210, 136.600, 178.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.700 - 2.470
R-factor	0.2116
Rwork	0.210
R-free	0.24290
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.512
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	REFMAC
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	2.540
High resolution limit [Å]	2.470	2.470
Rmerge	0.156	0.778
Number of reflections	52173	219
<I/σ(I)>	13.3	3.5
Completeness [%]	59.4	2.7
Redundancy	13.7	13.9
CC(1/2)	0.998	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	10% PEG 4000, 0.1 M sodium citrate (pH 6.0), 0.2 M magnesium chloride, 1 mM methylthioadenosine