9N1A
crystal structure of diferric HrmI from Streptomyces griseoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-28 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.945, 112.945, 66.967 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.820 - 2.130 |
| R-factor | 0.1789 |
| Rwork | 0.176 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.826 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.820 | 2.206 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.173 | |
| Rmeas | 0.181 | |
| Number of reflections | 27795 | 2730 |
| <I/σ(I)> | 12.61 | 2.83 |
| Completeness [%] | 99.5 | 99.93 |
| Redundancy | 11.4 | 11.3 |
| CC(1/2) | 0.997 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | ammonium sulfate, HEPES pH 7.5 |
