9MUJ

RlmR 23S rRNA methyltransferase from Thermus thermophilus in complex with methylated rRNA (Um2552) and S-adenosyl-L-homocysteine (SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 2
Unit cell lengths	112.920, 117.110, 58.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.311 - 2.006
R-factor	0.2071
Rwork	0.206
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11_2563)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.311	47.311	2.060
High resolution limit [Å]	2.006	8.970	2.010
Rmerge	0.128	0.039	2.464
Rmeas	0.133	0.040	2.558
Number of reflections	52487	678	3823
<I/σ(I)>	11.92
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.999	0.999	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.5	292	Na-Citrate 0.1M, pH4.5, PEG6000 10%, LiCl 1M