9MTT

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site deacylated-tRNAcys at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.050, 450.800, 622.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	188.390 - 2.600
R-factor	0.2261
Rwork	0.224
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvl
RMSD bond length	0.004
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	188.390	188.390	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.216	0.054	2.595
Rmeas	0.229	0.058	2.762
Total number of observations	15303448
Number of reflections	1782124	20752	130239
<I/σ(I)>	9.18	32.1	0.9
Completeness [%]	99.9	98.6	99.1
Redundancy	8.587	8.689	8.17
CC(1/2)	0.995	0.996	0.128

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME