9MTR

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGS-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.80A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 21 21 21
Unit cell lengths	210.010, 450.190, 622.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.560 - 2.800
R-factor	0.2197
Rwork	0.217
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvl
RMSD bond length	0.004
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.560	69.560	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.242	0.029	1.337
Rmeas	0.282	0.034	1.555
Total number of observations	5005217
Number of reflections	1393193	15313	104124
<I/σ(I)>	6.21	30.53	1.01
Completeness [%]	97.5	90.5	99.1
Redundancy	3.593	3.503	3.65
CC(1/2)	0.966	0.998	0.219

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME