9MTQ

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGD-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	208.960, 448.050, 615.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.560 - 2.550
R-factor	0.2178
Rwork	0.216
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvl
RMSD bond length	0.004
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.560	122.560	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.149	0.033	1.596
Rmeas	0.170	0.037	1.832
Total number of observations	7750111
Number of reflections	1824998	21081	135670
<I/σ(I)>	8.01	27.5	0.9
Completeness [%]	98.6	96.6	99.5
Redundancy	4.247	4.498	4.033
CC(1/2)	0.993	0.998	0.204

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME